Calcium-dependent physiologic and pathologic stimulus-metabolic response coupling in hepatocytes

Intracellular calcium, currents, and stimulus-response coupling in endothelial cells.

Vascular endothelium appears to be a unique organ. It not only responds to numerous hormonal and chemical signals but also senses changes in physical parameters such as shear stress, producing mediators that modulate the responses of numerous cells, including vascular smooth muscle, platelets, and leukocytes. In many cases, the initial response of endothelial cells to these diverse signals invo...

Stimulus-response coupling: the search for intracellular calcium mediator proteins.

Tutorial in Molecular and Cellular Biology Intracellular Calcium, Currents, and Stimulus-Response Coupling in Endothelial Cells

Vascular endothelium appears to be a unique organ. It not only responds to numerous hormonal and chemical signals but also senses changes in physical parameters such as shear stress, producing mediators that modulate the responses of numerous cells, including vascular smooth muscle, platelets, and leukocytes. In many cases, the initial response of endothelial cells to these diverse signals invo...

Stimulus - response coupling in human platelets

The role of changes in cytoplasmic free calcium, [Ca2 + ]J,in the responses to plateletactivating factor (PAF) was studied in human platelets loaded with the fluorescent calcium indicator, quin2. In the presence of 1 mm external calcium, PAF raised [Ca2 +]i 8-10-fold in a few seconds to peak near 1 pm. [Ca2 + ]I, then declined over several minutes towards the basal level. In the absence of exte...

Ouabain increases the calcium concentration in intracellular stores involved in stimulus-response coupling in human platelets.

The effect of ouabain on Ca2+ homeostasis in human platelets was studied using both quin 2 and chlorotetracycline to monitor changes in cytosolic Ca2+ as well as changes in the amount of Ca2+ accumulated in intracellular storage sites. In resting platelets, ouabain induces a concentration- and time-dependent increase in cytosolic Ca2+ concentration and a marked elevation of Ca2+ in the intracel...